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标题：Assessment of Mutations of Ha- and Ki-ras Oncogenes and the p53 Suppressor Gene in Seven Malignant Mesothelioma Patients Exposed to Asbestos
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作者：Fumihiko KITAMURA ; Shunichi ARAKI ; Takeshi TANIGAWA 等
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：1998
卷号：36
期号：1
页码：52-56
DOI：10.2486/indhealth.36.52
出版社：National Institute of Occupational Safety and Health
摘要：To examine whether malignant mesothelioma due to asbestos has genetic alterations in the Ha- and Ki-ras oncogenes or in the p53 suppressor gene, we analyzed the point mutations of these genes in paraffin-embedded autopsy samples of the primary tumors of malignant mesothelioma in seven asbestos patients who died from malignant mesothelioma. The genetic analysis was conducted by the polymerase chain reaction-single strand comformation polymorphysms (PCR-SSCP) method in all patients, and through the sequencing of deoxyribonucleic acid (DNA) bases in one patient. No genetic alterations were found in exons 1 or 2 of Ha- and Ki-ras oncogenes, or in exons 5 to 9 of the p53 gene, in any of the patients. Further studies on a larger number of patients are required to reach a definite conclusion concerning the genetic effects of asbestos on malignant mesothelioma.
关键词：Asbestos;Malignant mesothelioma;Paraffin-embedded tissue;ras oncogene;p53 suppressor gene;PCR-SSCP analysis;Sequencing analysis