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标题：Involvement of Caspase 3 Mediated Apoptosis in Hematopoietic Cytotoxicity of Metabolites of Ethylene Glycol Monomethyl Ether
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作者：Akira TAKAGI ; Takayuki YAMADA ; Koji HAYASHI 等
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：2002
卷号：40
期号：4
页码：371-374
DOI：10.2486/indhealth.40.371
出版社：National Institute of Occupational Safety and Health
摘要：The hematopoietic toxicity of ethylene glycol monomethyl ether (EGME) and its metabolites, methoxy acetaldehyde (MALD) and methoxyacetic acid (MAA), was analyzed using human bone marrow cells from a lymphoma patient without bone marrow involvement and a human leukemia cell line, HL 60. After 24-hour incubation, the concentrations of 50 percent inhibition (IC₅₀) of human hematopoietic progenitor cells with MALD or MAA were 3 mM and 3.9 mM, respectively, and EGME (10 mM or more) did not show any cytotoxicity. IC₅₀ (after 48-hour exposure) of MALD and MAA on HL 60 cells were 2.45 mM and 5.6 mM, respectively, suggesting that both hematopoietic progenitor cells and HL60 have a similar sensitivity. DNA ladder formation, a characteristics of apoptosis, was observed in MALD- or MAA-treated HL60 cells, but not in EGME-treated samples. Caspase-3 enzyme activity, the effector of the apoptotic process, was greatly enhanced with MALD treatment. The inhibitor of caspase-3 repressed cell death induced with MALD as well as MAA.
关键词：Ethylene glycol monomethyl ether;Apoptosis;Hematopoiesis;Caspase-3