文章基本信息

标题：Integrated Biomonitoring of Dioxin-like Compounds for Waste Management and Environment
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作者：Shin-ichi SAKAI ; Hidetaka TAKIGAMI
期刊名称：Industrial Health
印刷版ISSN：0019-8366
电子版ISSN：1880-8026
出版年度：2003
卷号：41
期号：3
页码：205-214
DOI：10.2486/indhealth.41.205
出版社：National Institute of Occupational Safety and Health
摘要：Many of the biological and toxic responses of polychlorinated dioxins and furans (PCDD/Fs) have been shown to be mediated through the aryl hydrocarbon receptor (AhR). Several bioanalytical methods have been developed to analyze Ah receptor agonists in cost-and-time effective way such as CALUX-bioassay (chemical-activated luciferase gene expression). This bioassay can detect the sum of dioxin-like activity (including unknown substances) in complex waste and environmental samples. The present paper gives a description of the bioanalytical method and application examples to analyze dioxin-like compounds in waste management samples of thermal/chemical dechlorination processes. The current detection limit of the CALUX in rat hepatoma H4IIE recombinant cells is less than 1 pM TEQ (0.06 pg TEQ/well). The CALUX bioassay is considered to be useful tools for the rapid and integrated monitoring of environmental emission and residual samples as a pre-screening or in combination to chemical analysis.
关键词：Dioxin;Bioassay;Ah receptor;CALUX;Waste management;Incineration ly ash;Thermal dechlorination;PCB;Chemical treatment