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标题：Fatty Acid Composition and Arachidonate Metabolites in the Livers of Ethanol-Treated Rats Fed an Arachidonate-Supplemented Diet: Effect of Dietary Fat
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作者：Misako OKITA ; Takayo SASAGAWA ; Kazuhko SUZUKI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1998
卷号：44
期号：6
页码：745-756
DOI：10.3177/jnsv.44.745
出版社：Center for Academic Publications Japan
摘要：The effects of 1% arachidonic acid ethyl ester (AA) ad-ministration on the liver prostanoid metabolites and on serum and liver lipids in 3g/kg ethanol-administered rats fed 10% lard or corn oil were studied. The rats were divided into 6 groups: lard-sucrose (Lard-Suc); lard-ethanol without AA (Lard-Et); lard-ethanol with AA (Lard-EtAA); corn oil-sucrose (Corn-Suc); corn oil-ethanol without AA (Corn-Et); and corn oil-ethanol with AA (Corn-EtAA). Liver triglyceride increased in Corn-EtAA compared with Corn-Et. Arachidonic acid (20:4 n -6) levels in liver phospholipid were significantly decreased in Corn-Et, but elevated in Lard-Et. The levels of 20:4 n -6 were significantly increased with AA administration in both ethanol groups. Liver 6-keto-prostaglandin F1α (6-keto-PGF1α) in Corn-Suc (24.7±5.1 pg/mg protein) was markedly higher than in Lard-Suc (4.5±1.2pg/mg protein), and the 6-keto-PGF1α lowered significantly with the addition of ethanol (9.3±0.9pg/mg protein), but it increased with AA administration (21.6±4.9pg/mg protein). In Lard-EtAA, a significant increase in 6-keto-PGF1α was observed compared with Lard-Suc. The liver leukotriene B₄ (LTB₄) level in Lard-Suc was significantly lower than that of Corn-Suc. In the corn oil group, ethanol feeding was associated with a significant increase in liver LTB₄. AA administration to Corn-Et suppressed the elevated LTB₄. Serum thiobarbituric acid-reactive substance (TBARS) concentrations in the corn oil group were higher than in the lard group, and these concentrations were not altered by AA administration. From these results, we concluded that the administration of AA in rats treated with ethanol increased 20:4 n -6 in liver phospholipid and liver PGI₂ levels, irrespective of dietary fat, and may protect against alcoholic liver injury. AA with a diet rich in linoleic acid (18:2_n-6), however, may increase fat in the alcoholic liver.
关键词：ethanol fed rats;arachidonic acid ethyl ester;dietary fat;6-keto-prostaglandin F1α;leukotriene B4