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标题：In Vitro and In Vivo Effects of Exogenous Nucleotides on the Proliferation and Maturation of Intestinal Epithelial Cells
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作者：Norifumi SATO ; Taku NAKANO ; Hiroshi KAWAKAMI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1999
卷号：45
期号：1
页码：107-118
DOI：10.3177/jnsv.45.107
出版社：Center for Academic Publications Japan
摘要：To determine the nutritional role of nucleotides, the in vitro and in vivo effects of exogenous nucleotides on the development of intes-tine were investigated. First, the in vitro effects of nucleotides on the proliferation and maturation of enterocytes were studied by using a human colon tumor cell line (Caco-2) and a rat normal small intestinal crypt cell line (IEC-6). Second, the in vivo effects of nucleotides were also studied in early weaned rats fed nucleotide-unsupplemented or high-nucleotide-supplemented diet. Nucleotide composition resembled that of human milk (CMP:UMP:AMP:IMP:GMP=10:1:1:1:1, in weight). Nucleotide supplement did not enhance Caco-2 cells proliferation; however, it sig-nificantly enhanced maltase and sucrase activities. In contrast, nucleo-tides supplement enhanced IEC-6 cells proliferation and maltase activity. CMP, predominantly contained in the mixture, enhanced most effectively the proliferation and maturation of cells. In the in vivo experiment, nu-cleotides significantly enhanced sucrase activity in the intestinal mucosa of early weaned rats. The results presented here suggest that a nucleotide supplement may enhance enterocyte proliferation and/or maturation in vivo and in vitro. Therefore exogenous nucleotides may play an impor-tant role in the development of the intestine.
关键词：Caco-2 cells;IEC-6 cells;nucleotides;intestinal develop-ment;early weaned rat