文章基本信息

标题：Variability in α-Tocopherol Antioxidant Activity in the Core and Surface Layers of Low-and High-Density Lipoproteins
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作者：Roberta CAZZOLA ; Giovanna CERVATO ; Benvenuto CESTARO 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1999
卷号：45
期号：1
页码：39-48
DOI：10.3177/jnsv.45.39
出版社：Center for Academic Publications Japan
摘要：The effect of α-tocopherol enrichment of low- and high-density lipoproteins on Cu²⁺-catalyzed lipid peroxidation in the hydrophobic core and in the hydrophilic envelope of lipoproteins was investigated by using two pyrene derivatives, namely, cholesteryl pyrenyl hexanoate (P₆Chol) and pyrene dodecanoyl sulfatide (P₁₂CS). The progressive decrease in fluorescence of P₆Chol was used to monitor lipid peroxidation in the core of LDL and HDL, whereas that of P₁₂CS was used to follow lipid peroxidation in the envelope of both lipoproteins. α-Tocopherol enrichment of LDL and HDL was obtained by incubating blood plasma at 37°C with different concentrations of the vitamin (25-500μM) before lipoprotein separation. The incorporation of α-tocopherol in LDL and HDL presents a progressive, time-dependent increase up to 200μM α-tocopherol, then a plateau up to 500μM. In the envelopes, the added tocopherol causes a great decrease in the rate of peroxidation and a dramatic increase in the latency phase in both lipoproteins. In the cores the lengthening of latency phase resulting from α-tocopherol enrichment was by far greater in LDL than in HDL, and the decrease in the rate of peroxidation in both lipoproteins was less than in the envelopes.
关键词：lipid peroxidation;lipoproteins;pyrene lipids;α-tocopherol;atherosclerosis