文章基本信息

标题：Formation of 8, 11, 14-Octadecatrienoic Acid (18:3 n-4) from Naturally Occurring Unique Fatty Acid, 9, 12-Hexadecadienoic Acid (16:2 n-4), in Animal Cell Cultures
本地全文：下载
作者：Nobuhiro NAKANO ; Norifumi SHIRASAKA ; Tetsuo MURAKAMI 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1999
卷号：45
期号：2
页码：203-212
DOI：10.3177/jnsv.45.203
出版社：Center for Academic Publications Japan
摘要：9, 12-Hexadecadienoic acid (16:2 n -4), present in small amounts in fish oils as a naturally occurring unique fatty acid, was incorporated into the phospholipids in rat liver BRL-3A cells to a similar extent as linoleic acid (18:2 n -6). 11, 14-Octadecadienoic acid (18:2 n -4) and 8, 11, 14-octadecatrienoic acid (18:3 n -4) were detected in the cellular lipids of BRL-3A cells when incubated in a medium supplemented with 16:2 n -4 methyl ester. The cellular levels of these acids increased in parallel with 16:2 n -4 methyl ester added to the medium. These compounds were probably formed through conversion from 16:2 n -4 to 16:3 n -4 by d 6 desaturation, and then 18:3 n -4 was produced by elongation, and part of the surplus 16:2 n -4, not desaturated to 16:3 n -4, elongated to 18:2 n -4. These results suggested that 16:2 n -4 was desaturated by d6 desaturase in vitro. It was also shown that 16:2 n -4 inhibited arachidonic acid synthesis from exogenous linoleic acid in BRL-3A cells as efficiently as a-linolenic acid (18:3 n -3).
关键词：9,12-hexadecadienoic acid;11,14-octadecadienoic acid;8,11,14-octadecatrienoic acid;desaturation;animal cell culture