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标题：Inhibitory Effect of Cladosiphon Fucoidan on the Adhesion of Helicobacter pylori to Human Gastric Cells
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作者：Hideyuki SHIBATA ; Itsuko KIMURA-TAKAGI ; Masato NAGAOKA 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：1999
卷号：45
期号：3
页码：325-336
DOI：10.3177/jnsv.45.325
出版社：Center for Academic Publications Japan
摘要：We studied the inhibitory effect of Cladosiphon fucoidan on the attachment of Helicobacter pylori ( H. pylori ), a gastroduodenal pathogen, to human gastric cell lines. The bacterial binding in these cell lines was inhibited more by Cladosiphon fucoidan (IC₅₀=16-30mg/mL), than by the fucoidan from Fucus (IC₅₀>30mg/mL). Dextran sulfate, another sulfated polysaccharide, did not inhibit the binding at all. Pre-incubating the bacterial suspension with fucoidans reinforced the inhibitory ability of these components, and reduced the IC₅₀ value of Cladosiphon fucoidan to approximately 1mg/mL. However, the binding was not inhibited by pre-treatment of gastric cells with these components. It was also shown that this fucoidan blocks both Le^b-and sulfatide-mediated attachment of H. pylori to gastric cells. Furthermore, fucoidan-binding proteins were found on the H. pylori cell surface by Western blot analysis. Thus, the inhibitory effect exerted by Cladosiphon fucoidan on binding between H. pylori and gastric cells might result from the coating with this component of the bacterial surface.
关键词：Helicobacter pylori;fucoidan;Cladosiphon okamuranus TOKIDA;gastric cell