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标题：Forms of Vitamin B12 Compounds Containing SulfitoB12 in Corbiculas
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作者：Keiko MIZOGUCHI ; Mamiko KAGAWA ; Mikio NAKAMURA 等
期刊名称：Journal of Nutritional Science and Vitaminology
印刷版ISSN：0301-4800
电子版ISSN：1881-7742
出版年度：2000
卷号：46
期号：2
页码：97-100
DOI：10.3177/jnsv.46.97
出版社：Center for Academic Publications Japan
摘要：Forms of vitamin B₁₂ (B₁₂) compounds in young and aged corbiculas were ex-amined by bioautography using B₁₂-requiring microorganisms combined with cellulose ac-etate membrane electrophoresis or high-performane liquid chromatography (HPLC). Both corbiculas (per 100 g) contained ca. 30 ug of cobalamin, a complete type of B₁₂. Five known B₁₂ compounds, adenosylB₁₂(AdoB₁₂), methylB₁₂, hydroxoB₁₂, sulfitoB₁₂ and cyanoB₁₂, were identified by bioautography. Young corbicula contained more B₁₂ compounds, as-sumed as methylB₁₂, AdoB₁₂ and sulfitoB₁₂, as compared to aged corbicula. All of the B₁₂ compounds detected around the location corresponding to those of methylB₁₂, AdoB₁₂ and sulfitoB₁₂ in the bioautography were converted into compounds that behaved like hydroxoB₁₂ after photolysis. Young corbicula was found to contain an unidentified B₁₂ com-pound using bioautography combined with HPLC. A large portion of the B₁₂ compound that moved like hydroxoB₁₂ during cellulose acetate membrane electrophoresis using 0.5 N acetic acid might be identical with the unidentified B₁₂ compound detected in the HPLC-bioautog-raphy.
关键词：vitamin B12;forms of vitamin B12;sulfitoB12;corbicula