文章基本信息

标题：Effect of Cupric Ion on Cholesteryl Ester Hydrolysis in Rat Peritoneal Macrophages
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作者：Mitsuo TANAKA
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：2
页码：125-127
DOI：10.1248/bpb.16.125
出版社：The Pharmaceutical Society of Japan
摘要：The inhibitory effects of Cu²⁺ on the hydrolysis of cholesteryl [4-¹⁴C]oleate droplets in rat peritoneal macrophages were studied. Macrophages rapidly incorporated cholesteryl [4-¹⁴C]oleate droplets and hydrolyzed at a linear rate over 9h.When macrophages were preincubated in the medium containing Cu²⁺, the hydrolysis of cholesteryl oleate within macrophages was markedly inhibited by Cu²⁺ and eventually led to the accumulation of cholesteryl oleate in macrophages. The inhibitory effect on the hydrolysis of cholesteryl oleate was dependent on the concentration of Cu²⁺. Also, the activity of acid cholesteryl ester hydrolase (acid CEH) in lysosomal fractions isolated from rat peritoneal macrophages with Cu²⁺ showed a marked decrease. This decrease also occurred in a dose dependent manner.These results suggest that Cu²⁺ inhibits the hydrolysis of cholesteryl ester in macrophages, and that the accumulation of cholesteryl ester in macrophages of the early atheroscleotic lesion is responsible for the decrease of lysosomal acid CEH activity.
关键词：cholesterol metabolism;acid cholesteryl ester hydrolase;lysosome;macrophage;cupric ion