文章基本信息

标题：Characterization of Tumor Necrosis Factor Alpha-Induced Alteration of Glycosaminoglycans in Cultured Cells : Comparison among Vascular Smooth-Muscle Cells, Vascular Endothelial Cells, Chang Liver Cells and LLC-PK1 Cells
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作者：Toshiyuki KAJI ; Syouichi HIRAGA ; Chika YAMAMOTO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：9
页码：834-839
DOI：10.1248/bpb.16.834
出版社：The Pharmaceutical Society of Japan
摘要：We investigated the alteration of glycosaminoglycans (GAGs) induced by recombinant human tumor necrosis factor alpha (rhTNFα) using confluent cultures of bovine aortic smooth-muscle cells, bovine aortic endothelial cells, Chang liver cells and porcine kidney LLC-PK1 cells. It was found that the incorporation of both [³⁵S]sulfate and [³H]glucosamine into GAGs in the trypsinate fraction of the cell layer was significantly decreased by rhTNFα in vascular smooth-muscle cells and vascular endothelial cells; the incorporation of [³⁵S]sulfate was increased but that of [³H]glucosamine was unchanged in Chang liver cells; the incorporation of both [³⁵S]sulfate and [³H]glucosamine was increased by rhTNFα in LLC-PK1 cells. In the conditioned medium, the incorporation of both [³⁵S]sulfate and [³H]glucosamine was not greatly changed by rhTNFα in all tested cell types. Characterization of GAGs revealed that each cell type uniquely altered its GAGs after rhTNFα treatment; the cytokine-induced alteration of each GAG component was not necessarily the same among different cell types. It was therefore concluded that rhTNFα-induced alteration of GAGs is dependent upon cell type.
关键词：cytokine;endothelial cell;glycosaminoglycan;tumor necrosis factor alpha;vascular;vascular smooth-muscle cell