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标题：Biological Activity and Structural Characterization of Alkaline-Soluble Polysaccharides from the Kernels of Prunus mume SIEB. et ZACC.
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作者：Chikaku DOGASAKI ; Hajime MURAKAMI ; Motohiro NISHIJIMA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1994
卷号：17
期号：3
页码：386-390
DOI：10.1248/bpb.17.386
出版社：The Pharmaceutical Society of Japan
摘要：The fruits of Prunus mume SIEB. et ZACC. (Japanese name, ume) have been used as a traditional drug and health food. In order to study the active components of P. mume, the polysaccharide fractions were extracted with cold water, hot water and aqueous sodium hydroxide from the kernels of P. mume. We found that some of the polysaccharide fractions exhibited various types of biological activities such as mitogenesis, activation of the alternative pathway of complement and activation of clot formation in human plasma. A polysaccharide, P-1, obtained from the cold 0.5 M NaOH extract was purified by ion-exchange chromatography and gel-filtration. P-1 contained 62.0% neutral sugar as glucose and 38.4% uronic acid (as galacturonic acid), and was free from protein. The neutral sugars of P-1 were arabinose, xylose, rhamnose and galactose in a molar ratio of 9.4 : 3.4 : 1.1 : 1.0, following analysis by gas-liquid chromatography. In addition, galacturonic acid was identified by thin-layer chromatography. The molecular weight of P-1 was found to be more than 2000000 by gel-filtration on Toyopearl HW 65F. P-1 showed mitogenic activity towards spleen cells of both C3H/HeN and C3H/HeJ, suggesting that it was free from bacterial endotoxic lipopolysaccharides.
关键词：Prunus mume kernel;heteroglycan;biological activity;arabinose;galacturonic acid;mitogen