文章基本信息

标题：Kinetic Studies on a Sulfotransferase from Klebsiella K-36, a Rat Intestinal Bacterium
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作者：DongHyun KIM ; HyungSoo KIM ; Lisa IMAMURA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1994
卷号：17
期号：4
页码：543-545
DOI：10.1248/bpb.17.543
出版社：The Pharmaceutical Society of Japan
摘要：Sulfotransferase purified from Klebsiella K-36, a rat intestinal bacterium, stoichiometrically catalyzed the transfer of a sulfate group of phenylsulfate esters to phenolic compounds. One of the reaction products, p-nitrophenol (PNP), non-competitively inhibited the enzyme as to p-nitrophenylsulfate (PNS), a donor substrate, but competitively inhibited the enzyme as to an acceptor substrate, α-naphthol. The other reaction product, α-naphthol-O-sulfate, non-competitively inhibited the enzyme with regard to both these substrates. These kinetic data suggest that the sulfotransferase reaction proceeds according to an ordered bi bi reaction mechanism. The natural phenolic substances. gallic acid, quercetin, tannic acid, and serotonin were good substrates of K-36 sulfotransferase.
关键词：sulfotransferase;sulfoconjugation;Klebsiella;intestinal bacteria;reaction mechanism