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标题：Structural Analysis of Alkaline-Soluble Polysaccharide, P-1, from the Kernels of Prunus mume SIEB. et ZUCC.
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作者：Chikaku DOGASAKI ; Hajime MURAKAMI ; Motohiro NISHIJIMA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1995
卷号：18
期号：3
页码：377-381
DOI：10.1248/bpb.18.377
出版社：The Pharmaceutical Society of Japan
摘要：A polysaccharide fraction extracted with cold 0.5M NaOH from the kernels of Prunus mume exhlbited some biological activities. A polysaccharide, P-1, was purified from the 0.5M NaOH extract by ion-exchange chromatography and gel-flltration. The results of the structural analysis of P-1 to determine the relationship between the activities and the structure are described in this paper. In the mild acid hydrolysis of P-1, the nondialyzable hydrolysate (I-3) believed to be its core portion was obtained. The yield of I-3 was 26.0% and contained 59.8% uronic acid as galacturonic acid (GalA). The neutral sugars of I-3 were composed of rhamnose, xylose and galactose in a molar ratio of 1.0 : 3.4 : 0.3 following analysis by gas-liquid chromatography. The molecular weight of I-3 was estimated to be ca. 14000 by gel-filtration on Toyopearl HW55F. I-3 exhibited the mitogenic activity toward spleen cells as well as P-1. These facts appeared to confirm that I-3 was the core part of P-1 and important for its biological activity. I-3 was successfully reduced by the Taylor and Conrad method to avoid so much repetition. Methylation analysis of the reduced hydrolysate by gas-liquid chromatography and gas chromatography-mass spectroscopy showed that the ratio of 1, 4-linked galactopyranosyl and 1, 3, 4-linked galactopyranosyl residues were significantly increased in comparison with native I-3. These results suggested that I-3 was composed of 1, 4- and 1, 3, 4-linked galacturonic acid residues in the main chain.
关键词：Prunus mume kernel;polysaccharide;controlled hydrolysis;uronic acid;methylation analysis;mitogenic activity