文章基本信息

标题：Induction of Unresponsiveness of Antigen-Specific T Lymphocytes by Oral Administration of Cedar Pollen Extract in Mice
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作者：Yasuhiro KOHAMA ; Hideki TONE ; Kazuki MORIYAMA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1995
卷号：18
期号：10
页码：1315-1319
DOI：10.1248/bpb.18.1315
出版社：The Pharmaceutical Society of Japan
摘要：T lymphocyte unresponsiveness, induced in mice by a single gastric intubation of 0.2 ml cedar pollen extract (CPE, containing 4μg protein/ml)/mouse daily for 3 to 28 consecutive days, was evaluated by the absence of a proliferative response of popliteal lymph node (PLN) T lymphocytes to CPE in vitro. T lymphocyte unresponsiveness increased with the period of gastric intubation of CPE and reached more than 80% of the control on day 28. The unresponsiveness to CPE was antigen-specific and T lymphocyte-mediated. In vitro CPE-specific T lymphocyte proliferation was significantly suppressed by intestinal intraepithelial lymphocytes (IELs) and hepatic mononuclear cells (MNCs), but not by spleen cells or PLN T lymphocytes from mice fed CPE for 28 days. The effector activity of IELs and MNCs was obviously antigen-specific. These results suggest that lymphocytes in the intestine and liver of mice fed CPE would be involved in the induction and maintenance of CPE-specific T lymphocyte unresponsiveness.
关键词：oral tolerance;T lymphocyte unresponsiveness;cedar pollen;intestinal intraepithelial lymphocyte;hepatic mononuclear cell;T lymphocyte proliferation