文章基本信息

标题：Transcriptional Induction of Acyl-CoA Oxidase by Dehydroepiandrosterone Sulfate in Cultured Rat Hepatocytes
本地全文：下载
作者：Junji YAMADA ; Hiroyuki SUGIYAMA ; Takafumi WATANABE 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：4
页码：630-631
DOI：10.1248/bpb.19.630
出版社：The Pharmaceutical Society of Japan
摘要：To elucidate the molecular basis of the induction of peroxisomal enzymes, transcriptional regulation of the acyl-CoA oxidase (AOX) gane by dehydroepiandrosterone sulfate (DHEAS) was examined using primary cultures of rat hepatocytes. Incubation of the hepatocytes with 0.1 mM DHEAS resulted in a progressive increase in the cellular AOX mRNA level, which after 24 h reached a 5.6-fold higher level than that in the control cells treated with vehicle alone. However, this mRNA induction was completely inhibited by α-amanitin, an RNA polymerase II inhibitor. When examined by the nuclear run-on transcription assay, the rate of de novo synthesis of AOX mRNA was 3- to 4-fold higher in the hepatocytes exposed to DHEAS than in the control cells, even after 3 h. These results indicate that DHEAS acts directly on hepatocytes to activate the transcription of the AOX gene, which leads to the marked induction of AOX in the cell.
关键词：dehydroepiandrosterone;peroxisome proliferator;enzyme induction;transcriptional activation;rat hepatocyte culture