文章基本信息

标题：Phenobarbital Molecularly Imprinted Polymer Selectively Binds Phenobarbital
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作者：Yoshihisa TOMIOKA ; Yoshiki KUDO ; Tetsuro HAYASHI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：4
页码：397-400
DOI：10.1248/bpb.20.397
出版社：The Pharmaceutical Society of Japan
摘要：Molecularly imprinted polymer (MIP) was propared against phenobarbital using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as the cross linking monomer. We analyzed the recognition properties of the phenobarbital MIP. In some organic solvents, imprinted polymer showed selective binding to phenobarbital. Two dissociation constants of binding were calculated by Scatchard plot analyses; K_d values were 1.8, 121.7 μM, and the number of binding sites was 8.3, 92.3 μmol/g MIP in toluene-heptane-acetic acid (25 : 75 : 1, v/v), respectively. The relationship between the binding affinity to phenobarbital MIP and the polarity of the solvent system, as well as the structure of the template molecule is also discussed.
关键词：molecularly imprinted polymer (MIP);molecular imprinting;phenobarbital;molecular recognition;antibody mimic