文章基本信息

标题：Carbonyl Reductase Activity Exhibited by Pig Testicular 20β-Hydroxysteroid Dehydrogenase
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作者：Shizuo NAKAJIN ; Fumihiro TAMURA ; Noriko TAKASE 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：11
页码：1215-1218
DOI：10.1248/bpb.20.1215
出版社：The Pharmaceutical Society of Japan
摘要：The carbonyl reductase activity exhibited by pig testicular 20β-hydroxysteroid dehydrogenase (20β-HSD) was examined using a recombinant enzyme. Kinetic parameters were obtained for 48 carbonyl group-containing substrates, including aromatic aldehydes, aromatic ketones, cycloketones, quinones, aliphatic aldehydes and aliphatic ketones. 20β-HSD showed a high affinity towards quinones, such as 9, 10-phenanthrenequinone, α-naphthoquinone and menadione (K_m values of 4, 2 and 5 μM, respectively), and the substrate utilization efficiency (V_max/K_m) of the enzyme against these quinones was very high. Cyclohexanone and 2-methylcyclohexanone were also reduced with a high V_max/K_m value, but not cyclopentanone or 2-methylcyclopentanone. Various aromatic aldehydes and ketones including benzaldehyde- and acetophenone-derivatives were reduced by 20β-HSD. Especially, 4-nitrobenzaldehyde and 4-nitroacetophenone were reduced with high V_max/K_m values in related compounds. The enzyme also reduced the pyridine-derivatives, 2-, 3-, and 4-benzoylpyridine, with the V_max/K_m value for 2-benzoylpyridine being the highest. 20β-HSD reduced aliphatic aldehydes and aliphatic ketones, but was more effective on the former. The correlation between the structure of carbonyl compounds and their substrate V_max/K_m is discussed.
关键词：20β-hydroxysteroid dehydrogenase;testis;pig;carbonyl reductase;kinetics;recombinant enzyme