文章基本信息

标题：Phylogenetic Analysis of Atractylodes Plants Based on Chloroplast trnK Sequence
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作者：Hajime MIZUKAMI ; Ryosuke SHIMIZU ; Mareshige KOHJYOUMA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1998
卷号：21
期号：5
页码：474-478
DOI：10.1248/bpb.21.474
出版社：The Pharmaceutical Society of Japan
摘要：The phylogenetic relationship of Atractylodes lancea, A. chinensis, A. koreana, A. ovata and A. japonica were analyzed by comparing the 2.6 kb sequence in a chloroplast gene trnK encoding tRNA^Lys (UUU). The dried rhizomes of the former three species have been used as the crude drug "So-jutsu" and those of the latter two as "Byaku-jutsu" in Chinese and Japanese traditional medicine ("Kampo-medicine"). The trnK phylogenetic tree revealed that A. ovata is an outgroup of the five Atractylodes species examined and that A. japonica and A. lancea are most closely related. PCR amplification of trnK with HinfI digestion provided us with a simple method to distinguish A. ovata from other Atractylodes species at the molecular level.
关键词：molecular phylogeny;Atractylodes;chloroplast gene;trnK sequence;PCR-RFLP