文章基本信息

标题：Hepatic Disposition Characteristics of Recombinant Human Interleukin-11 (rhIL-11) in the Perfused Rat Liver
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作者：Akira TAKAGI ; Yoshiyuki YABE ; Mitsunobu YOSHIDA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1998
卷号：21
期号：12
页码：1364-1366
DOI：10.1248/bpb.21.1364
出版社：The Pharmaceutical Society of Japan
摘要：The hepatic disposition characteristics of recombinant human interleukin-11 (rhIL-11) were investigated in perfused rat liver to clarify the mechanism of hepatic clearance which is a major contributor to the rapid clearance of rhIL-11 in vivo. We analyzed the disposition characteristics of [¹¹¹In]-labeled rhIL-11 using a single-pass constant infusion mode at different concentrations of rhIL-11. The venous outflow rapidly reached a steady-state condition at every concentration. Liver extraction ratio at steady-state (E_SS) was decreased with increase in the concentration, suggesting that there is a saturable interaction between the liver cells and rhIL-11 molecule. Cellular localization experiments demonstrated that rhIL-11 was taken up by both liver parenchymal and non-parenchymal cells depending on their surface area, suggesting that this uptake was mediated by electrostatic interaction due to cationic charges in the cytokine.
关键词：recombinant human interleukin-11 (rhIL-11);hepatic disposition;rat liver perfusion;cationic macromolecule