文章基本信息

标题：Participation of Cytosolic Protein Phosphatase in Regulation of NADPH Oxidase in Polymorphonuclear Leukocytes
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作者：Naoko KAWAKAMI ; Hiroaki TAKEMASA ; Naoki OKAMURA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：6
页码：556-560
DOI：10.1248/bpb.22.556
出版社：The Pharmaceutical Society of Japan
摘要：Calyculin A, a protein phosphatase inhibitor, enhanced phorbol 12-myristate 13-acetate (PMA)-induced superoxide anion (O^-₂) producuction and translocation of the cytosolic NADPH oxidase factor, p47phox, to the plasma membrane in guinea pig polymorphonuclear leukocytes (PMNs). When PMNs were treated with 1-(5-isoquinoline-sulfonyl)-3-methyl-piperazine (H-7), a protein kinase C (PKC) inhibitor, after exposure to PMA, inhibition of O^-₂ production and translocation of p47phox to the membrane fraction in PMA-stimulated PMNs were observed. When calyculin A was added to the PMA-stimulated PMNs after the addition of H-7, O^-₂ production was again observed, and translocation of p47phox to the membrane fraction also occurred. The activity of NADPH oxidase, the amount of p47phox and the level of phosphorylation of p47phox in the membrane fraction prepared form PMA-stimulated PMNs, were reduced by the addition of the cytosol fraction from unstimulated PMNs.These reductions were attenuated by calyculin A. These results indicated that the active from of NADPH oxidase in PMNs can be reconstituted after the active complex of the enzyme has disappeared once, and that one of the mechanisms of regulation of this enzyme activity involves the phosphorylation of p47phox in the cyotosol and dephosphorylation of phosphorylated p47phox in the NADPH oxidase complex by protein kinase and protein phosphatase, respectively.
关键词：polymorphonuclear leukocyte;NADPH oxidase;calyculin A;protein phosphatase