文章基本信息

标题：New Metabolic Pathway of (24R)-24, 25-Dihydroxyvitamin D3 : Epimerization of the 3-Hydroxy Group
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作者：Tatsuya HIGASHI ; Ryuta KIKUCHI ; Kanako MIURA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：7
页码：767-769
DOI：10.1248/bpb.22.767
出版社：The Pharmaceutical Society of Japan
摘要：A new metabolic pathway of (24R)-24, 25-dihydroxyvitamin D₃ [24, 25(OH)₂D₃] was clarified in the vivo experiments. After the administration of 24, 25(OH)₂D₃ to rats, a new monoglucuronide of a vitamin D metabolite was obtained from the bile together with 24, 25(OH)₂D₃ 3- and 24-glucuronides. The genin of the metabolite was identified as 3-epi-24, 25(OH)₂D₃ in comparison with the synthetic sample based on the data from ¹H-NMR, GC/MS, and LC/atmospheric pressure chemical ionization-MS. The conjugation position was determined to be the 24-hydroxy group by the LC/electrospray ionization-MS and -MS/MS/MS combined with derivatization. To our knowledge, this is the first reported instance of the epimerization of the 3-hydroxy group of vitamin D compound with no hydroxy group at the 1α-position.
关键词：3-epi-24, 25-dihydroxyvitamin D3;in vivo metabolite;synthesis;LC/MS;glucuronide