文章基本信息

标题：Adhesive Interaction of Highly Malignant Hepatoma AH66F Cells with Mesothelial Cells
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作者：Masaaki NOMURA ; Yuhki SUGIURA ; Yasuaki TATSUMI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：7
页码：738-740
DOI：10.1248/bpb.22.738
出版社：The Pharmaceutical Society of Japan
摘要：We investigated the mechanism of adhesion of highly malignant ascites hepatoma AH66F cells to mesothelial cells. The adhesion rate of AH66F cells to mesentery-derived mesothelial cells (M-cells) was about 46% at 37°C, but it decreased to about 27% at 4°C. The adhesion rate of AH66F cells was about 25% in the presence of leukocyte function-associated antigen 1 (LFA-1) mAb at both 4°C and 37°C. When M-cells were treated with hyaluronidase, the AH66F/M-cell adhesion was decreased to half at 37°C and had nearly disappeared at 4°C. The residual adhesion of AH66F cells to M-cells treated with hyaluronidase almost disappeared in the presence of LFA-1 mAb. AH66F cells strongly adhered to a hyaluronate (HA)-coated plate, but not to a bovine serum albumin-coated plate. AF66F cells expressed a CD44 molecule (a HA receptor) in the plasma membrane, with a molecular size of about 85 to 90 kDa, corresponding to the CD44H isoform.These results indicated that the adhesion of AH66F cells to mesothelial cells is composed of pathways of CD44/HA and LFA-1/ICAM-1.
关键词：rat hepatoma;leukocyte function-associated antigen 1 (LFA-1);CD44;hyaluronan;mesothelial cell