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标题：Hypericin Induces Both Differentiation and Apoptosis in Human Promyelocytic Leukemia HL-60 Cells
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作者：Kyung-Tae LEE ; Joo-Il KIM ; Young-Soo RHO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：12
页码：1271-1274
DOI：10.1248/bpb.22.1271
出版社：The Pharmaceutical Society of Japan
摘要：Hypericin is a unique photosensitizing plant pigment and has been separately reported to induce differentiation and apotosis in neoplastic cells. In this study, we examined the relationship between activities to induce differentiation and apoptosis in human promyelocytic leukemia HL-60 cells, at a concentration range of 0.15 to 0.2μM. When treated with hypericin, the cell ratio reducible of nitroblue tetrazolium was significantly increased and the cell size was enlarged by flow cytometry analysis. Hypericin also significantly increased the ratio of the cells, which were of positive α-naphthyl acetate esterase activity and phagocytic activity, whereas it hardly influenced the naphthol AS-D chloroacetate esterase activity in the cells, as well as 1α, 25(OH)₂D₃ (10nM). In addition, hypericin increased hypodiploid nuclei and caused a nucleosomal ladder. These results indicate that hypericin induced both differentiation toward monocyte/macrophage lineage and apoptosis in HL-60 cells.
关键词：hypericin;differentiation;apoptosis;HL-60 cell