文章基本信息

标题：p-Aminohippurate Transport in Rat Renal Brush-Border Membranes : a Potential-Sensitive Transport System and an Anion Exchanger
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作者：Kou OHOKA ; Mikihisa TAKANO ; Tomonobu OKANO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：4
页码：395-401
DOI：10.1248/bpb.16.395
出版社：The Pharmaceutical Society of Japan
摘要：Transport mechanisms of p-aminohippurate (PAH) were investigated in rat renal brush-border membrane vesicles. The uptake of PAH was stimulated by an inside-positive membrane potential created by K⁺ and valinomycin. This potential-stimulated uptake of PAH was inhibited by various anion transport inhibitors and was saturable. In addition, PAH uptake in the presence of valinomycin was linearly increased in proportion to log[K⁺]_out/[K⁺]_in. On one hand, PAH uptake was stimulated by [¹⁴C]PAH/PAH or [¹⁴C]PAH/Cl^- exchange, and the [¹⁴C]PAH/PAH exchange was insensitive to the membrane potential. The uptake by the exchanger was also inhibited by anion transport inhibitors, but the potential-stimulated uptake of PAH was more sensitive to furosemide and 4, 4'-diiso-thiocyano-2, 2'-disulfonic stilbene. On the contrary, [¹⁴C]PAH/PAH exchange was more sensitive to urate than the potential-stimulated uptake of PAH. These findings indicate that PAH is transported by two distinct transport systems in rat renal brush-border membranes, a potential-sensitive transport system and an anion exchanger.
关键词：p-aminohippurate;renal secretion;brush-border membrane vesicle;organic anion;membrane potential;anion exchange