文章基本信息

标题：Restriction Fragment Length Polymorphisms (RFLPs) of Medicinal Plants and Crude Drugs. I. RFLP Probes Allow Clear Identification of Duboisia Interspecific Hybrid Genotypes in Both Fresh and Dried Tissues
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作者：Hajime MIZUKAMI ; Kaoru OHBAYASHI ; Yoshie KITAMURA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：4
页码：388-390
DOI：10.1248/bpb.16.388
出版社：The Pharmaceutical Society of Japan
摘要：A simple and efficient method for identification of Duboisia leichhardtii F. MUELL, D. myoporoides R. BR. and their interspecific hybrid was established by analyzing restriction fragment length polymorphism (RFLP) profiles using non-radioactive rice ribosomal DNA as a probe. This procedure was shown to be applicable to both fresh and dried samples, and also suitable for detection of somatic hybrid at the callus stage after protoplast fusion.
关键词：restriction fragment length polymorphism (RFLP);hybrid identification;Duboisia;Solanaceae;leaf;callus