文章基本信息

标题：Inhibition of the Induction and Activity of Hepatic P450IA Isozymes by in Vivo Administration of Carbon Tetrachloride to Rats
本地全文：下载
作者：Masakuni DEGAWA ; Ken-ichi MIKAMI ; Masayuki NAMIKI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：12
页码：1248-1250
DOI：10.1248/bpb.16.1248
出版社：The Pharmaceutical Society of Japan
摘要：The effect of carbon tetrachloride (CCl₄) on the induction and activity of 3-methylcholanthrene (MC)-inducible P450IA isozymes (P450IA1 and P450IA2) in the liver was examined by treating male Sprague-Dawley rats with simultaneous i.p. injections of MC and CCl₄. Following the treatment, the quantities of P450IA mRNAs determined by Northern blotting and P450IA proteins by Western blotting were significantly reduced as compared with those in rats treated with MC alone. As determined by a bacterial mutation test using carcinogenic aromatic amines are P450IA enzyme substrates, CCl₄ treatment selectively inhibited the activity of P450IA2 in the MC-induced isozymes. These results indicate that CCl₄ exerts on inhibitory effect on the induction of P4501A isozymes and that it selectively inhibits P450IA2 activity.
关键词：cytochrome P450;P450IA isozyme;carbon tetrachloride;rat;liver