文章基本信息

标题：Responses of Glutathione-Related Enzymes in Isolated Rat Small Intestine to Fe2+-EDTA-Mediated Oxidative Stress
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作者：Tadashi MIZOGUCHI ; Yoshiaki MORITA ; Hirofumi NANJO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1994
卷号：17
期号：5
页码：607-611
DOI：10.1248/bpb.17.607
出版社：The Pharmaceutical Society of Japan
摘要：The activities of glutathione-related enzymes in isolated rat small intestine were investigated under oxidative stress mediated by Fe²⁺-EDTA. The isoelectric points and approximate molecular weights of the enzymes investigated were first determined. The reduced and oxidized glutathione contents, and low molecular weight thiols in isolated rat small intestine were also studied under oxidative stress. Significant reducing activities of glutathione S-transferase and lactate dehydrogenase were observed accompanied by increases in oxidized glutathione content, whereas thioltransferase, glutathione reductase, glutathione peroxidase and thioredoxin reductase retained the same levels of activity as controls. First-order inactivation of purified rat small intestime glutathione S-transferase including class-α, μ and π was observed and the isozyme class-π was inactivated at several pH's under Fe²⁺-EDTA-mediated oxidative stress. Leakage of protein and both reduced and oxidized forms of glutathione was significantly increased during incubation with Fe²⁺-EDTA. In conclusion, enzymes which were not inactivated under Fe²⁺-EDTA-mediated oxidative stress may play an important role in cellular antioxidant defenses in the small intestine. Furthermore, enzymes such as glutathione S-transferase, mainly a class-π isozyme, and lactate dehydrogenase which were inactivated may form part of the barrier against oxidative stress similar to reduced glutathione.
关键词：oxidative stress;glutathione-related enzyme;Fe2+-EDTA;rat small intestine;glutathione S-transferase