文章基本信息

标题：Autoradioluminography, a Novel Quantitative Method of TLC-Autoradiography
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作者：Mitsunobu OKUYAMA ; Yasuhiko HATORI ; Akiyo SHIGEMATSU 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1994
卷号：17
期号：5
页码：559-563
DOI：10.1248/bpb.17.559
出版社：The Pharmaceutical Society of Japan
摘要：In pharmacokinetic studies, ordinary methods of identifying or quantitatively measuring metabolites have been complicated and become difficult to handle. Here, a novel TLC-autoradioluminography (TLC-ARLG) is reported. This technique is especially suited for quantitatively measuring a minute amount, below-1 ng, of many radioactive metabolites in plasma, tissues or organs, thus eliminating cumbersome extraction procedures. The method has an extremely wide dynamic range and could offer a powerful analytical technique for studying a radioactive compound and its metabolites in a biological specimen. Autoradioluminography is a new quantitative technology developed by combining autoradiography with radioluminography. Radioluminography is a kind of radiography in which a specially designed radio-sensor plate, called an "Imaging plate (IP), "developed by Fuji Photo Film Co., Ltd., is used. The radio-sensor is made of fine crystals of BaF : Eu²⁺, and the technology is based on photo-luminescence phenomena.
关键词：quantitative TLC-autoradioluminography;imaging plate;metabolic study