文章基本信息

标题：High-Performance Liquid Chromatographic Determination of Human Serum Albumin in Plasma and Urine by Post-column Fluorescence Enhancement Detection Using 8-Anilino-1-naphthalenesulfonic Acid
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作者：Junichi ISHIDA ; Kazutomo ABE ; Masaru NAKAMURA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1996
卷号：19
期号：11
页码：1391-1395
DOI：10.1248/bpb.19.1391
出版社：The Pharmaceutical Society of Japan
摘要：A method for the quantitative determination of human serum albumin in plasma and urine by post-column high-performance liquid chromatography with fluorescence detection is presented. Human serum albumin in plasma and urine is separated by gel-permeation column chromatography, which is followed by fluorescence detection. The detection uses the fluorescence enhancement observed when 8-anilino-1-naphthalenesulfonic acid binds to human serum albumin. The method is rapid to perform and is highly sensitive (detection limit is 2.5 ng/injection volume at a signal-to-noise ratio of three).
关键词：human serum albumin;HPLC;fluorescence;8-anilino-1-naphthalenesulfonic acid