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标题：Primary Structure of Base Non-specific and Acid Ribonuclease from Bullfrog (Rana catesbeiana)
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作者：Norio INOKUCHI ; Hiroko KOBAYASHI ; Makiko MIYAMOTO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：5
页码：471-478
DOI：10.1248/bpb.20.471
出版社：The Pharmaceutical Society of Japan
摘要：A base non-specific acid ribonuclease (RNase RCL₂) was purified from bullfrog liver [H. Yagu et al. Biol. Pharm. Bull., 18, 219-222 (1992)]. The sequence study and comparison of the amino acid sequence of the enzyme with homologous RNases from oyster, drosophila and chicken liver suggested that the RNase RCL₂ consisted of two components, large protein fraction (182 amino acid residues) and peptide 2 (20 amino acid residues) or peptide 1 (18 amino acid residues), and that both components bind with disulfide bridge. The RNase preparation was probably formed from single polypeptide protein by processing with some proteses. The amino acid sequence of RNase RCL₂ showed that the RNase belongs to the RNase of RNase T₂ family and its sequence most resembles chicken liver acid RNase. In RNase RCL₂, the amino acid residues which sonsist of the active site and major base recognition site of RNase Rh, a typical RNase of RNase T₂ family, are very well conserved except for Tyr57 (RNase Rh numbering), and part of the amino acid residues of the minor base recognition site (Phe101 and Pro92) are also conserved.
关键词：bullfrog;base non-specific ribonuclease;primary structure;acid ribonuclease;phylogenetic relationship;Rana catesbeiana