文章基本信息

标题：Application of Glutaraldehyde-Crosslinked Chitosan as a Scaffold for Hepatocyte Attachment
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作者：Masaya KAWASE ; Nobuyasu MICHIBAYASHI ; Yumiko NAKASHIMA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：6
页码：708-710
DOI：10.1248/bpb.20.708
出版社：The Pharmaceutical Society of Japan
摘要：The effectiveness of chitosan, a biocompatible polymer derived by the deacetylation of chitin, as a scaffold of hepatocyte attachment, was examined. Since chitosan gel was too fragile to use for cell culture, its free amino groups were crosslinked by glutaraldehyde to increase its strength. Rat hepatocytes seeded onto glutaraldehyde-crosslinked chitosan (GA-chitosan) gel could stably sttach to the surface, retaining its spherical form, the same as in vivo, and the release a vary small amount of lactate dehydrogenase during the 5 d culture period. By contrast, hepatocytes on a collagen-coated surface spread flat, and they released much more lactate dehydrogenase than those on the GA-chitosan gel. Hepatocytes on GA-chitosan also retained higher urea synthesis activity, a liver-specific function, than those on the collagen-coated surface. These results indicate that chitosan is a promising biopolymer as a scaffold of hepatocyte attachment, which can be applied to an effective bioartificial liver support system.
关键词：chitosan;bioartificial liver;hepatocytes