文章基本信息

标题：Determination of the Functional Domain of a Mouse Autonomous Replicating Sequence
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作者：Chiharu HAYASHI ; Hiromichi FUJINO ; Masanori OGATA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：6
页码：690-693
DOI：10.1248/bpb.20.690
出版社：The Pharmaceutical Society of Japan
摘要：We previously isolated from mouse cells an autonomous replicating sequence (ARS) ARS65 (Ariga, Itani and Iguchi-Ariga, Mol. Cell. Biol. 7, 1-6, 1987). Here we report the nucleotide sequence of ARS65. The sequence from BglII to EcoRI sites cloned as ARS was 2568 bp long. There exist three intersting domains : a TA repeat, a myc like box (essential sequence for c-myc ARS, and a T rich region. Cloned DNAs containing various segments of pARS65 were transfected to rat 3Y1 cells together with the hydromycinB resistance expression vector, and hygromycinB resistant clones were isolated. Established cell lines transfected with plasmids carrying either a myc-like box or a T rich region harbored the replicated plasmids, indicating that these two elements are necessary for the ARS function of pARS65.
关键词：ARS;c-myc;replication