文章基本信息

标题：In Vitro Glucuronidation of 25-Hydroxyvitamin D3 and Its Pro-form
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作者：Kazutake SHIMADA ; Yuko KAMEZAWA ; Kuniko MITAMURA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1997
卷号：20
期号：6
页码：596-600
DOI：10.1248/bpb.20.596
出版社：The Pharmaceutical Society of Japan
摘要：In vitro glucuronidation of 25-hydroxyvitamin D₃ and its pro-form has been investigated by means of HPLC with UV detection. Although both substrates gave 3- and 25-glucuronides in the presence of the ray liver microsomal fraction and uridine-5'-diphosphoglucuronic acid, 25-hydroxyvitamin D₃ and its pro-form yielded 3- and 25-glucuronide as the main product, respectively. The latter glucuronide is the one in which the tert-hydroxy group is conjugated. Each glucuronide was identified by its chromatographic behavior in comparison with an authentic sample and data obtained from liquid chromatography/mass spectrometry (LC/MS) using atmospheric pressure chemical ionization.
关键词：25-hydroxyvitamin D3;25-hydroxyprovitamin D3;enzymic glucuronidation;substrate specificity;LC-MS