文章基本信息

标题：Effect of Extracellular Calcium on the Intracellular Calcium Level of Newborn Rat Skin Basal Cells
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作者：Kazuto OHKURA ; Hitoshi HORI ; Hiroshi TERADA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1998
卷号：21
期号：1
页码：1-4
DOI：10.1248/bpb.21.1
出版社：The Pharmaceutical Society of Japan
摘要：The effect of extracellular calcium on the intracellular calcium level of newborn rat skin basal cells was examined. The intracellular calcium level of basal cells was affected by the extracellular calcium concentration on culture of the cells on a collagen-coated filter, but not on a plate. The intracellular calcium ion concentration ([Ca²⁺]_i) of cells on a collagen-coated filter with 0.05mM external CaCl₂ was 15nM and increased to 20-22nM on culture with 2.0mM extracellular CaCl₂. From these results we concluded that the culture matrix (e.g. culture plates, or collagen-coated filters) affects the response of the basal cells to changes in the extracellular calcium content.The intracellular calcium level of Swiss 3T3 cells cultured both on plates and collagen-coated filters were affected by the extracellular calcium concentration. Their [Ca²⁺]_i was determined as 49-50nM in the presence of 0.05mM external CaCl₂, and 54-56nM in the presence of 2.0mM CaCl₂. These different responses to extracellular Ca²⁺ may be due to differences in the proliferative profiles of basal cells and fibroblasts.
关键词：skin;calcium;basal cell;epidermal cell