文章基本信息

标题：Trapping of Secreted Molecules at Single-Cell Level Using an HPLC Resin Particle Under a Videomicroscope
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作者：Tsutomu MASUJIMA ; Kayo IKEDA ; Koichiro OZAWA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1998
卷号：21
期号：8
页码：886-888
DOI：10.1248/bpb.21.886
出版社：The Pharmaceutical Society of Japan
摘要：Particles of HPLC resins are used for the trapping of secreted molecules from a single cell. The basic molecules, e.g., histamine, are secreted from a single RBL-2H3 cell by granule exocytosis and are trapped by cation-exchange HPLC resins outside the cell. Since quinacrine is concentrated into the exoctotic and acidic micro-granules in RBL-2H3 cells, which are used as a model cell line of mast cells, we measured the change in the fluorescence intensity of the quinacrine released from the cells and that of molecules trapped on the resin using a videomicroscope. By measuring the increase in the fluorescence intensity of te resins, we can estimate the real time course of molecular secretion from a single cell.
关键词：videoanalysis;HPLC resins;videomicroscopy;quinacrine;RBL-2H3 cells;A23187