文章基本信息

标题：S-(1, 2-Dicarboxyethyl)glutathione in Yeast : Partial Purification of Its Synthesizing Enzyme
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作者：Seiji TSUBOI ; Kunihiko KIYONO ; Bun-ichiro ONO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：1
页码：21-25
DOI：10.1248/bpb.22.21
出版社：The Pharmaceutical Society of Japan
摘要：S-(1, 2-Dicarboxyethyl)glutathione (DCE-GS) was found in Saccharomyces cerevisiae, but not in bacterial species nor in a unicellular alga (Acetabularia acetabulm). The enzyme that catalyzes condensation of L-malate and glutathione (GSH) to form DCE-GS was partially purified from baker's yeast. It had a molecular mass of 49 kDa and was monomeric and the K_m values were 2.2 and 1.4 mM for L-malate and GSH, respectively. The enzyme had a pH optimum of 7.5. DCE-GS levels in yeast cells were significantly higher in aerobic cultures than in anaerobic ones. DCE-GS was synthesized in cells cultured between 20 and 35°C.
关键词：Saccharomyces cerevisiae;S-(1, 2-dicarboxyethyl)glutathione;physiological role;synthesizing enzyme