文章基本信息

标题：A Vanadyl Sulfate-Bovine Serum Albumin Complex Stimulates the Release of Lipoprotein Lipase Activity from Isolated Rat Fat Pads through an Increase in the Cellular Content of cAMP and myo-Inositol 1, 4, 5-Trisphosphate
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作者：Toshio MOTOYASHIKI ; Mikoto MIYAKE ; Aya YOSHIDA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：8
页码：780-786
DOI：10.1248/bpb.22.780
出版社：The Pharmaceutical Society of Japan
摘要：A vanadyl sulfate-bovine serum albumin complex (vanadyl-BSA) prolonged the stability of the V⁴⁺ oxidation state, although vanadyl alone can readily change the oxidation state from V⁴⁺ to V⁵⁺ under physiological conditions. Vanadyl-BSA stimulated the release of lipoprotein lipase (LPL) acitivty from isolated rat fat pads and increased the cellular LPL activity in a time-dependent manner. These effects were independent of protein synthesis. Propranolol, quin 2-AM, ruthenium red, and neomycin all inhibited LPL release more potently than the increase in activity. In contrast, potent inhibition of the increase effect was observed with genistein and wortmannin. Short-term incubation of the fat pads with vanadyl-BSA showed a transient increase in the cellular content of cAMP and myo-inositol 1, 4, 5-triphosphate (IP₃), which was inhibited by propranolol and neomycin, respectively. These results suggest that vanadyl-BSA stimulates the release of LPL activity through an increase in the cellular content of cAMP and IP₃, leading to an increased intracellular Ca²⁺ concentration, and tat it also increases cellular LPL activity via process(es) sensitive to genistein and wortmanni.
关键词：vanadyl sulfate;lipoprotein lipase;calsium;cAMP;myo-inositol 1, 4, 5-trisphosphate