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标题：Arginyl-tRNA-Protein Transferase Activities in Crude Supernatants of Rat Tissues
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作者：Koichi TAKAO ; Keijiro SAMEJIMA
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：9
页码：1007-1009
DOI：10.1248/bpb.22.1007
出版社：The Pharmaceutical Society of Japan
摘要：A fluorescent HPLC method for the assay of arginyl-tRNA-protein transferase (R-Transferase) activity was applied to obtain quantitative data of the enzyme activity in rat tissues for the first time. In this assay, the major problem was a significant hydrolysis of the substrate, N-aspartyl-N'-dansylamido-1, 4-butanediamine, and the product, N-arginylaspartyl-N'-dansylamido-1, 4-butanediamine (ArgAsp(4)DNA) by aminopeptidases in crude samples such as 105000 g supernatants (105S) of tissue homogenates. As bestatin inhibited the hydrolysis of ArgAsp(4)DNA, a standard-addition method in the presence of bestatin, using a partially purified R-Transferase preparation from hog kidney as a standard, made it possible to measure directly R-Transferase activities in 105S with a short incubation time and sufficient reliability. It was found by the established method that of 14 tissues examined, stomach was rich in the R-Transferase activity with the highest specific activity, suggesting a target tissue for the future studies on R-Transferase to elucidate its physiological significance.
关键词：arginyl-tRNA-protein-transferase;fluorescent HPLC method;enzyme activity;rat tissue