文章基本信息

标题：Determination of Valproic Acid in Human Serum by High-Performance Liquid Chromatography with Fluorescence Detection
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作者：Shuuji HARA ; Motoki KAMURA ; Kazuo INOUE 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1999
卷号：22
期号：9
页码：975-977
DOI：10.1248/bpb.22.975
出版社：The Pharmaceutical Society of Japan
摘要：A simple and highly sensitive high-performance liquid chromatographic method is described for the determination of valproic acid in human serum. The method is based on the direct derivatization of serum sample with 6, 7-methylenedioxy-1-methyl-2-oxo-1, 2-dihydroquinoxaline-3-ylpropionohydrazide. The derivatization reaction proceeds in aqueous solution in the presence of pyridine and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide at 37°C. The resulting derivatives were separated on a reversed-phase column (YMC Pack ODS-A) with isocratic elution and were fluorimetrically detected at 440 nm with excitation at 365 nm. The detection limit (signal-to-noise ratio=3) for valproic acid added to serum sample was 0.1 μg (700 fmol)/ml serum sample (2.3 fmol on column). The method was applied to determine the unbound- and total-valproic acid levels in the serum obtained from three healthy volunteers after oral administration of the drug (600 mg).
关键词：valproic acid;HPLC;fluorescence detection;6, 7-methylenedioxy-1-methyl-2-oxo-1, 2-dihydroquinoxaline-3-ylpropionohydrazide;unbound