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标题：Evaluation of L-Glutamate Clearance Capacity of Cultured Rat Cortical Astrocytes
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作者：Kazuho ABE ; Yuzuru ABE ; Hiroshi SAITO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2000
卷号：23
期号：2
页码：204-207
DOI：10.1248/bpb.23.204
出版社：The Pharmaceutical Society of Japan
摘要：Astrocytes have a function in the uptake of excitatory neurotransmitter L-glutamate via the glutamate transporter. To evaluate the L-glutamate clearance capacity of atrocytes, we developed a colorimetric method for the determination of L-glutamate concentration and measured changes in extracellular L-glutamate concentration in rat cortical astrocyte cultures. When L-glutamate (50-200 μM) was added to astrocyte cultures and incubated for 1-8 h, the extracellular L-glutamate concentration declined with time. When L-glutamate was mixed with astrocyte culture supernatants only, no significant change in L-glutamate concentration was observed, ruling out the possibility that L-glutamate is spontaneously or enzymatically degraded in the extracellular space. Alternatively, the time-dependent decline of extracellular L-glutamate concentration was blocked by the presence of glutamate uptake inhibitors, indicating that the glutamate uptake system of astrocytes plays a major role in the clearance of extracellular L-glutamate.
关键词：L-glutamate;colorimetric measurement;astrocyte;glutamate transporter;culture