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标题：Quantification of a Very Low Level of Serum Human Immunodeficiency Virus Type 1 (HIV-1) RNA Using Competitive PCR at Intervals and the Clinical Course
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作者：Hitoraka NAITOU ; Jun-ichi MIMAYA ; Yasuo HORIKOSHI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2000
卷号：23
期号：3
页码：368-370
DOI：10.1248/bpb.23.368
出版社：The Pharmaceutical Society of Japan
摘要：Quantification of human immunodeficiency virus type 1 (HIV-1) genomic RNA levels below the detection limit (400 copies/ml) of commercially available quantification kits is possible by increasing the amount of serum samples using competitive PCR conditions. We evaluated disease prognosis in patiets without symptoms for a long period after infection (long-term non-progressor) and patients in whom the virus was controlled to a low level by administration of anti-HIV drugs. For 102 of the 414 serum samples stored at -80°C which showed HIV-1 RNA below 400 copies/ml by competitive PCR using 100 to 200 μl sera, an increase in the sample volume to 500-2000 μl, extraction of HIV-1 RNA, and quantitative detection by competitive PCR was performed. Follow-up quantification of serum HIV-1 RNA from 4 patients indicate that this method is useful in assessing prognosis in the early stage of the disease in patients without symptoms for a long period after infection (long-term non-progressor) and patients in whom the virus in the serum was controlled by administration of anti-HIV drugs to below the detection limit of commercially available quantification kits. Quantification of low level serum HIV-1 RNA was also considered useful as a parameter of treatment.
关键词：low level serum HIV-1 RNA detection;quantitative PCR;competitive PCR;anti-HIV therapy evaluation;disease prognosis