文章基本信息

标题：Separation of Phosphatidylinositol 4-Phosphate from a Mixture with Phosphatidylserine and Phosphatidylinositol by Sep-Pak C18 Cartridge
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作者：Naoko MORISAKI ; Miho MASAOKA ; Ryuichi SHIRAI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：2000
卷号：23
期号：9
页码：1088-1089
DOI：10.1248/bpb.23.1088
出版社：The Pharmaceutical Society of Japan
摘要：A simple and efficient method for the separation of phosphatidylinositol 4-phosphate (PI 4-P) from phosphatidylinositol (PI) and phosphatidylserine (PS) is described. A mixture of PI, PI 4-P and PS was injected onto a Sep-Pak C₁₈ cartridge. PI and PS were flushed through the cartridge with solvent 1 [methanol-chloroform (3 : 1)] while PI 4-P remained in it. Then the cartridge was inverted, and PI 4-P was eluted backward with solvent 2 [chloroform-methanol-0.5 M aqueous ammonium hydroxide (9 : 7 : 2)].
关键词：Sep-Pak C18 cartridge;phosphatidylinositol 4-phosphate;phosphatidylinositol;phosphatidylinositol 3-phosphate;phosphatidylserine