文章基本信息

标题：Recognition of a Palindromic DNA Sequence by ESF-1, a Factor Stimulating Transcription of the Adenovirus Type 12 E1A Gene
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作者：Hitomi SAKURAI-SHIBATA ; Yukito MASAMUNE ; Yoshinobu NAKANISHI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：1
页码：87-89
DOI：10.1248/bpb.16.87
出版社：The Pharmaceutical Society of Japan
摘要：We previously identified a factor, designated E1A-stimulating factor 1 (ESF-1), that stimulates transcription of the adenovirus type 12 E1A gene in vitro by binding to a region next to a TATA box. We report here on the partial characterization of the mode of ESF-1 action. Point mutations within the nucleotide sequence 5'-TCAGCTGA-3' abolished the binding of ESF-1. Stimulation of transcription of the adenovirus type 12 E1A gene in vitro was lost when a 7 base pair insertion was introduced between the ESF-1-binding site and a TATA box. These results suggest that ESF-1 stimulates adenovirus type 12 E1A gene transcription by interacting with a palindromic DNA sequence present in a region immediately upstream from a TATA box.
关键词：adenovirus;transcriptional regulation;DNA-protein interaction;cell-free transcription;cis-acting element;cultured cell