文章基本信息

标题：Adriamycin-Induced Lipid Peroxidation of Erythrocyte Membranes in the Presence of Ferritin and the Inhibitory Effect of Ceruloplasmin
本地全文：下载
作者：Toshiaki MIURA ; Sanae MURAOKA ; Taketo OGISO 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：7
页码：664-667
DOI：10.1248/bpb.16.664
出版社：The Pharmaceutical Society of Japan
摘要：When erythrocyte membranes were incubated with adriamycin (ADM) in the presence of ferritin, lipid peroxidation occurred wiht release of iron from the ferritin. In the presence of apoferritin, ADM did not cause lipid peroxidation. Deferoxamine inhibited the ADM-induced lipid peroxidation in the presence of ferritin. These results indicate that lipid peroxidation depends upon the release of iron from ferritin. Even when the iron content in ferritin was very low, ADM could induce lipid peroxidaton. Superoxide dismutase, catalase and hydroxyl radical scavengers did not substantially affect lipid peroxidation, indicating that the peroxidation reaction was independent of superoxide, H₂O₂ and hydroxyl radicals. Ceruloplasmin, a ferroxidase, markedly inhibited lipid peroxidation but did not affect the release of iron from ferritin. ADM-Fe³⁺-binding erythrocyte membranes were readily formed during the incubation of erythrocyte membranes with ADM in the presence of ferritin, and deferoxamine removed iron from the ADM-Fe³⁺-binding membranes, indicating that the iron moiety of the ADM-Fe³⁺ complex is exposed at the membrane surface. These results may suggest that the peroxidation reaction occurs in a site-specific manner.
关键词：adriamycin;lipid peroxidation;ferritin;ceruloplasmin;erythrocyte membrane