文章基本信息

标题：Decline in Plasma Membrane Ca2+-ATPase Activity and Increase in Cytosolic-Free Ca2+ Concentration of Endotoxin-Injected Mice Livers
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作者：Shuhei SAKAGUCHI ; Eiji TSUTSUMI ; Katsushi YOKOTA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1993
卷号：16
期号：8
页码：808-810
DOI：10.1248/bpb.16.808
出版社：The Pharmaceutical Society of Japan
摘要：The present study describes the activity of Ca²⁺-ATPase in liver plasma membrane and cytosolic-free Ca²⁺ concentration ([Ca²⁺]_i) in an individual vital cell in mouse liver 18h after endotoxin administration. Ca²⁺-ATPase activity in liver plasma membrane in the poisoned mice was markedly decreased to 28% of that in the control. The membrane protein damage in liver was found mostly in the molecular weight (M.W.) regions near 60000-150000 in endotoxemic mice, and was markedly injured near 140000 (M. W. of Ca²⁺-ATPase in liver plasma membrane). The level of [Ca²⁺]_i in liver cells in endotoxin-poisoned mice was greater at 18h postintoxication than that of the control.These findings suggest the possibility that the depression of Ca²⁺-ATPase activity in liver plasma membrane in mice may contribute to membrane damage caused by the endotoxin, and that the increase of [Ca²⁺]_i in liver cytoplasm may partially explain various endotoxin-induced metabolic disorders.
关键词：endotoxin;Ca2+-ATPase activity;liver plasma membrane damage;[Ca2+]i in liver cell