文章基本信息

标题：Synergistic Enhancement of Nitrite on Lysophospholipid-Mediated Cytolysis
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作者：Masahiro KANEMATSU ; Kuniaki TAKAGI ; Yasunobu SUKETA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1994
卷号：17
期号：1
页码：78-81
DOI：10.1248/bpb.17.78
出版社：The Pharmaceutical Society of Japan
摘要：Nitrogen oxide, which is produced by activated macrophages, has been demonstrated to possess anti-tumor activity. We report herein the synergistic effect of sodium nitrite (NO^-₂) and/or sodium nitroprusside (SNP) on lysophospholipid (LysoPL)-mediated cytolysis. The incubation of ⁵¹Cr-labeled mouse melanoma (B16) cells with NO^-₂ alone for 3 h at 37°C did not induce cytolysis. On the other hand, NO^-₂ significantly enhanced the cytolysis of B16 cells in the presence of lysophosphatidylcholine (LysoPC; 2.0 μM). A similar effect of NO^-₂ on B16-cytolysis was also observed in the presence of 1-O-alkyl-sn-glycero-3-phosphocholine (LysoPAF). In addition, SNP (0.05-0.5 mM) synergistically enhanced B16-cytolysis in the presence of LysoPC. However, nitrate had no effect on the cytolysis of B16 cells treated with LysoPC. Furthermore, NO^-₂ synergistically enhanced the hemolysis of sheep erythrocytes in the presence of LysoPC, but not in the presence of an anti-sheep erythrocyte antibody and complement. These findings suggest that NO^-₂ directly affects membrane damage in the presence of LysoPL.
关键词：nitric oxide;lysophospholipid;cytolysis