文章基本信息

标题：Radioimmunoassay of Conjugated 1β-Hydroxycholic Acid in Dried Blood Spots for Diagnosis of Congenital Biliary Atresia
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作者：Shigeo IKEGAWA ; Tsuyoshi MURAI ; Akira MATSUI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1994
卷号：17
期号：1
页码：5-8
DOI：10.1248/bpb.17.5
出版社：The Pharmaceutical Society of Japan
摘要：Radioimmunological measurement of conjugated 1β-hydroxycholic acid in dried blood spots obtained from newborns at 4-5 d after birth has been carried out to investigate a diagnostic utility for congenital biliary atresia. The method allowed the determination of this bile acid, with a detection limit of 50 pg in one disc (3 mm i.d.), and showed good reproducibility with inter- and intra-assay coefficients with variations of 3.5-7.7% and 6.3-8.8%. With the analysis, a relatively high concentration of this substance was observed in the patients, as compared to normal subjects, averaging 1.63 ng/disc in the former and 0.97 ng/disc in the latter.
关键词：radioimmunoassay;conjugated 1β-hydroxycholic acid;bile acid;dried blood disc;gas chromatography-mass spectrometry;congenital biliary atresia