文章基本信息

标题：Studies on Thermophile Products. VIII. Isolation of Bacillus stearothermophilus UBT8038, a Component That Inhibits Antigen Presentation on Mouse Macrophages
本地全文：下载
作者：Yasuhiro KOHAMA ; Masahiko ITOH ; Keishi TANAKA 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1994
卷号：17
期号：7
页码：889-893
DOI：10.1248/bpb.17.889
出版社：The Pharmaceutical Society of Japan
摘要：We have found a new compound from thermophile extracts which inhibits antigen presentation on mouse macrophages. The substance inhibits the expression of the class II major histocompatibility molecule (Ia). It was extracted from Bacillus stearothermophilus UBT8038 and purified by silica gel column chromatography. The isolated inhibitor, Fr. 8-A, was a phosphatidylethanolamine with isofatty acids and chemically different from any of the natural or synthetic products which have been reported to modify Ia expression. Fraction 8-A inhibits Ia expression by mouse peritoneal macrophages induced by the supernatant from concanavalin A stimulated spleen cell cultures in a dose-dependent fashion over the range 0.1-10 μg/ml. This fraction also exhibited inhibitory effects on antigen presentation by splenic macrophages in vitro and on the mixed leukocyte reaction. The present results show that Fr. 8-A has a unique inhibitory effect on antigen presenting cells.
关键词：Bacillus stearothermophilus;class II major histocompatibility molecule;antigen presenting cell;macrophage;mixed leukocyte reaction