文章基本信息

标题：A Visual Membrane Immunoassay for the Detection of Methamphetamine Using an Enzyme-Labeled Tracer Derived from Methamphetamine and Amphetamine
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作者：Myung Ja CHOI ; Boris M. GOROVITS ; Jeongeun CHOI 等
期刊名称：Biological and Pharmaceutical Bulletin
印刷版ISSN：0918-6158
电子版ISSN：1347-5215
出版年度：1994
卷号：17
期号：7
页码：875-880
DOI：10.1248/bpb.17.875
出版社：The Pharmaceutical Society of Japan
摘要：A visual membrane enzyme immunoassay is described for the measurement of methamphetamine in urine. To increase assay sensitivity, tracers with chemically similar structures were cross-checked with the antibodies to determine their influence on the antibody binding. Tracers of horseradish peroxidase-labeled methamphetamine (MA-HRP) and amphetamine (A-HRP) derivatives were prepared for this purpose. Significant differences in antibody specificity were found between the two tracers. Based on the results of this study, a pair of an antibody and a tracer was selected and a membrane enzyme immunoassay (EIA) was developed utilizing the competitive binding between methamphetamine and the drug-HRP tracer. UltraBind membrane (0.45μm) was used as the solid matrix to which the antibody was attached. Using diaminobenzidine substrate with Co²⁺ ion, a stable grey color appeared on the surface of membrane for MA-negative urine samples. No color appeared for MA-positive urine with a cut-off level of 0.8 ppm.
关键词：methamphetamine;amphetamine;immunogen;tracer influence;visual membrane immunoassay